Recent Advances in the Research on Luotonins A, B, and E

This digest review summarises the most recent progress in the study on luotonins A, B and E. The literature covered in this overview spans from January 2012 to April 2024 and presents synthetic methodologies for the assembly of the quinolinopyrrolo-quinazoline scaffold, the structural motifs present in luotonins A, B, and E, and the evaluation of the biological activities of their derivatives and structural analogues.


Introduction
Since the discovery of luotonins in the Peganum nigellastrum Bunge (Zygophyllaceae), there has been ongoing interest in the synthesis and biological evaluation of these intriguing compounds [1][2][3].Luotonins were isolated from the aerial parts of plants that have been long used in Chinese traditional medicine for treating rheumatism, abscesses, and diseases accompanied by inflammation.All isolated luotonin alkaloids (luotonins A-F) have shown moderate cytotoxic activity against selected cancer cell lines [4][5][6][7][8].Among them, luotonin A (1) displays promising cytotoxicity against murine leukaemia P-388 cells (IC 50 1.8 mg/mL) facilitated by stabilising the topoisomerase I and DNA complex [9], similar to the structurally related topo inhibitor, camptothecin [10] (Figure 1).Although this alkaloid is not sufficiently active for cancer chemotherapy, it has become an interesting lead structure.In this context, there is still ongoing interest in the construction of the quinolinopyrrolo-quinazoline scaffolds and related frameworks.Numerous successful synthetic approaches to this class of compounds have appeared over the last decades.In 2011, Jahng and coworkers [11] published an account of the biological activities and syntheses of luotonins A-F.The presented review covers the latest findings on the synthesis of luotonins A, B, and E as well as their derivatives and structural analogues and the evaluation of their biological activities over the period from January 2012 to April 2024.

Introduction
Since the discovery of luotonins in the Peganum nigellastrum Bunge (Zygophyllac there has been ongoing interest in the synthesis and biological evaluation of these i guing compounds [1][2][3].Luotonins were isolated from the aerial parts of plants that h been long used in Chinese traditional medicine for treating rheumatism, abscesses, diseases accompanied by inflammation.All isolated luotonin alkaloids (luotonins have shown moderate cytotoxic activity against selected cancer cell lines [4][5][6][7][8].Am them, luotonin A (1) displays promising cytotoxicity against murine leukaemia P-388 (IC50 1.8 mg/mL) facilitated by stabilising the topoisomerase I and DNA complex [9], ilar to the structurally related topo inhibitor, camptothecin [10] (Figure 1).Although alkaloid is not sufficiently active for cancer chemotherapy, it has become an interes lead structure.In this context, there is still ongoing interest in the construction of the q olinopyrrolo-quinazoline scaffolds and related frameworks.Numerous successful thetic approaches to this class of compounds have appeared over the last decades.In 2 Jahng and coworkers [11] published an account of the biological activities and synth of luotonins A-F.The presented review covers the latest findings on the synthesis of tonins A, B, and E as well as their derivatives and structural analogues and the evalua of their biological activities over the period from January 2012 to April 2024.Molecules 2024, 29, 3522.https://doi.org/10.3390/molecules29153522https://www.mdpi.com/journal/molecules

Synthesis and Biological Activity
The publications discussed in this review are divided according to the synthetic strategies used in constructing the pentacyclic structural core of the luotonins.
2.1.Synthesis of Luotonin A via Construction of the 5H-pyrrolo [4,3-b]pyridine Core (B and C Rings) The most frequently applied synthetic strategy for the construction of the quinolinop yrrolo-quinazoline skeleton during the reviewed period uses aza-Diels-Alder addition as a key step in the synthesis of target compounds.
Haider and coworkers [12][13][14] reported two synthetic pathways starting from ethyl 4-oxo-3,4-dihydroquinazoline-2-carboxylate 4, which allow the selective introduction of various substituents into all available positions of ring A of luotonin A. The first paper [12] describes the synthesis of luotonin A and its substituted derivatives by improving Yao's synthetic route starting from anthranilamide [15].The anilide-type key intermediates 5 were prepared from ethyl 4-oxo-3,4-dihydroquinazoline-2-carboxylate 4 in two steps by applying Weinreb's amidation method, providing products in good yields (Scheme 1).Subsequently, the [4+2] cycloaddition reaction of requisite intermediate 6, generated in situ from 5 by treatment with Hendrickson's reagent provided the pyrrolo [4,3-b]pyridine core (B and C rings of 1).Alternatively, the simultaneous assembly of B and C rings of the luotonin A skeleton was achieved from carbonitriles 8 [13] by employing Wang's conditions (heating in 1,2-dichlorobenzene at 110-120 • C in the presence of 5 mol % DBU) [16].This three-step sequence, DBU-mediated alkyne/allene isomerisation, intramolecular [4+2] cycloaddition reaction of allene 9, and a 1,5-hydrogen shift in the primary cycloadduct then produces the aromatic quinoline scaffold.Arylpropargyl-substituted quinazolinonecarbonitriles 8 were obtained by the Sonogashira coupling of appropriate aryl iodides with 4-oxo-3-propargyl-3,4-dihydroquinazoline-2-carboxamides 7 and the subsequent dehydration reaction of the amides.This method is suitable for the selective introduction of both electron-donating as well as electron-withdrawing substituents into position 1 or position 3 of the alkaloid compared to a previously used cycloaddition approach that gave access to 2or 4-substituted luotonin A derivatives (Scheme 1).

Synthesis and Biological Activity
The publications discussed in this review are divided according to the synthetic strategies used in constructing the pentacyclic structural core of the luotonins.

Synthesis of Luotonin A via Construction of the 5H-pyrrolo[4,3-b]pyridine Core (B and C Rings)
The most frequently applied synthetic strategy for the construction of the quinolinopyrrolo-quinazoline skeleton during the reviewed period uses aza-Diels-Alder addition as a key step in the synthesis of target compounds.
Haider and coworkers [12][13][14] reported two synthetic pathways starting from ethyl 4-oxo-3,4-dihydroquinazoline-2-carboxylate 4, which allow the selective introduction of various substituents into all available positions of ring A of luotonin A. The first paper [12] describes the synthesis of luotonin A and its substituted derivatives by improving Yao's synthetic route starting from anthranilamide [15].The anilide-type key intermediates 5 were prepared from ethyl 4-oxo-3,4-dihydroquinazoline-2-carboxylate 4 in two steps by applying Weinreb's amidation method, providing products in good yields (Scheme 1).Subsequently, the [4+2] cycloaddition reaction of requisite intermediate 6, generated in situ from 5 by treatment with Hendrickson's reagent provided the pyrrolo [4,3-b]pyridine core (B and C rings of 1).Alternatively, the simultaneous assembly of B and C rings of the luotonin A skeleton was achieved from carbonitriles 8 [13] by employing Wang's conditions (heating in 1,2-dichlorobenzene at 110-120 °C in the presence of 5 mol % DBU) [16].This three-step sequence, DBU-mediated alkyne/allene isomerisation, intramolecular [4+2] cycloaddition reaction of allene 9, and a 1,5-hydrogen shift in the primary cycloadduct then produces the aromatic quinoline scaffold.Arylpropargylsubstituted quinazolinonecarbonitriles 8 were obtained by the Sonogashira coupling of appropriate aryl iodides with 4-oxo-3-propargyl-3,4-dihydroquinazoline-2-carboxamides 7 and the subsequent dehydration reaction of the amides.This method is suitable for the selective introduction of both electron-donating as well as electron-withdrawing substituents into position 1 or position 3 of the alkaloid compared to a previously used cycloaddition approach that gave access to 2-or 4-substituted luotonin A derivatives (Scheme 1).

Scheme 1. Haiderʾs syntheses of luotonin A and its derivatives.
Preliminary in vitro screening (using the Resazurin assay [17]) for antiproliferative activity showed that thiophene isostere of luotonin A is practically inactive, whereas the 1,3-dimethoxy derivative exhibits a slightly higher activity profile than the lead Scheme 1. Haider's syntheses of luotonin A and its derivatives.
Later, Haider et al. [18,19] prepared a series of luotonin A analogues with modification to the A and E rings using the Povarov reaction as a key step (Figure 2).The evaluation of the antiproliferative activity towards human tumour cell lines revealed the 4-amino and 4,9-diamino compounds to be the most effective agents, showing an interesting profile of cytotoxic activity.Specifically, 4,9-diamino-luotonin A (IC 50 of 2.03 µM [95% CI = 1.79-2.29]against SW480 cells and an IC 50 of 0.82 µM [95% CI = 0.71-0.94]against HL60 cells) is a potent antiproliferative agent that is active in a similar concentration range as clinically used topo I inhibitors.
Molecules 2024, 29, x FOR PEER REVIEW 3 of 17 compound, luotonin A. The EC50 value of 1,3-dimethoxy-luotonin A towards the Ishikawa cell line (ECACC 99040201, endometrial carcinoma) is 5.84 µM (luotonin A: no inhibition), while the EC50 value of 1,3-dimethoxy derivative towards the MDA-MB-231 cell line (ATCC HTB-26, triple-negative breast cancer) is 31.82µM (luotonin A: >100 µM).The EC50 value of 1,3-dimethoxy derivate towards the other cell lines (KB/HeLa, NCI-H460, A2780, MDA-MB-435S) was >100 µM.Later, Haider et al. [18,19] prepared a series of luotonin A analogues with modification to the A and E rings using the Povarov reaction as a key step (Figure 2).The evaluation of the antiproliferative activity towards human tumour cell lines revealed the 4-amino and 4,9-diamino compounds to be the most effective agents, showing an interesting profile of cytotoxic activity.Specifically, 4,9-diamino-luotonin A (IC50 of 2.03 µM [95% CI = 1.79-2.29]against SW480 cells and an IC50 of 0.82 µM [95% CI = 0.71-0.94]against HL60 cells) is a potent antiproliferative agent that is active in a similar concentration range as clinically used topo I inhibitors.Luotonin A and its derivatives have also attracted attention as potential fungicides, insecticides [20], and pesticides [21].Liu and coworkers [20] reported on the fungicidal and insecticidal activity of halo-substituted derivatives of 1.The target compounds were synthesised from anthranilamide using Yao's synthetic strategy [15], applying an intramolecular aza-Diels-Alder reaction for the formation of the 5H-pyrrolo [4,3-b]pyridine core (B and C rings) (Scheme 2).To obtain more diversified modifications at the C-13 position of the pentacyclic skeleton, 1-methoxy luotonin A was treated with boron tribromide and subsequently subjected to a Mannich reaction with different amines to produce compounds 10a-10c.Luotonin A and its derivatives have also attracted attention as potential fungicides, insecticides [20], and pesticides [21].Liu and coworkers [20] reported on the fungicidal and insecticidal activity of halo-substituted derivatives of 1.The target compounds were synthesised from anthranilamide using Yao's synthetic strategy [15], applying an intramolecular aza-Diels-Alder reaction for the formation of the 5H-pyrrolo [4,3-b]pyridine core (B and C rings) (Scheme 2).To obtain more diversified modifications at the C-13 position of the pentacyclic skeleton, 1-methoxy luotonin A was treated with boron tribromide and subsequently subjected to a Mannich reaction with different amines to produce compounds 10a-10c.
Molecules 2024, 29, x FOR PEER REVIEW 3 of 17 compound, luotonin A. The EC50 value of 1,3-dimethoxy-luotonin A towards the Ishikawa cell line (ECACC 99040201, endometrial carcinoma) is 5.84 µM (luotonin A: no inhibition), while the EC50 value of 1,3-dimethoxy derivative towards the MDA-MB-231 cell line (ATCC HTB-26, triple-negative breast cancer) is 31.82µM (luotonin A: >100 µM).The EC50 value of 1,3-dimethoxy derivate towards the other cell lines (KB/HeLa, NCI-H460, A2780, MDA-MB-435S) was >100 µM.Later, Haider et al. [18,19] prepared a series of luotonin A analogues with modification to the A and E rings using the Povarov reaction as a key step (Figure 2).The evaluation of the antiproliferative activity towards human tumour cell lines revealed the 4-amino and 4,9-diamino compounds to be the most effective agents, showing an interesting profile of cytotoxic activity.Specifically, 4,9-diamino-luotonin A (IC50 of 2.03 µM [95% CI = 1.79-2.29]against SW480 cells and an IC50 of 0.82 µM [95% CI = 0.71-0.94]against HL60 cells) is a potent antiproliferative agent that is active in a similar concentration range as clinically used topo I inhibitors.Luotonin A and its derivatives have also attracted attention as potential fungicides, insecticides [20], and pesticides [21].Liu and coworkers [20] reported on the fungicidal and insecticidal activity of halo-substituted derivatives of 1.The target compounds were synthesised from anthranilamide using Yao's synthetic strategy [15], applying an intramolecular aza-Diels-Alder reaction for the formation of the 5H-pyrrolo [4,3-b]pyridine core (B and C rings) (Scheme 2).To obtain more diversified modifications at the C-13 position of the pentacyclic skeleton, 1-methoxy luotonin A was treated with boron tribromide and subsequently subjected to a Mannich reaction with different amines to produce compounds 10a-10c.

Scheme 2. Synthesis of luotonin A derivatives by Liu and Wang.
Novel luotonin A derivatives were tested for their activity against Botrytis cinerea, Magnaporthe oryzae, and Aphis craccivora.The results demonstrated that most of the luotonin A analogues displayed excellent in vitro biological activity against B. cinerea.Compared with the luotonin A (0.25 mM), 2-methoxy (0.18 mM), 2,3-dimethoxy (0.19 mM), 2,3-difluoro (0.17 mM), 10b (0.16 mM) and 10c (0.09 mM) compounds displayed the greatest antifungal activity.In particular, the in vitro antifungal activity of 1-morpholinomethyl derivative 10c is comparable to the positive control (azoxystrobin, 0.09 mM).The 3-fluoro and 3trifluoromethyl analogues of 1 were the most active compounds against A. craccivora, each showing the same mortality values of 42.05% at 50 mg/mL.
Wang and coworkers [21] evaluated some analogues of 1 for their antiviral activities and fungicidal activities.Natural luotonin A 1 displayed similar antiviral activity (inhibitory rates of 41%, 45%, and 36% at 500 µg/mL for inactivation, curative, and protection activities in vivo, respectively) to ribavirin (inhibitory rates of 40%, 37%, and 40% at 500 µg/mL for inactivation, curative, and protection activities in vivo, respectively).However, the effect of introducing both EWG and/or electron-donating groups to the A-ring on the activity is not obvious.The 2,3-dimethoxy and 2-chloro derivatives exhibited about similar levels of antiviral activities, while the activity of 2-methyl compounds was slightly lower.
Menéndez, Arumugam, Kumar, and coworkers [22,23] described the design, synthesis and biological study of a new class of luotonin A analogues 12 and 13, which differ from the parent compound by lacking the D-ring carbonyl substituent (Scheme 3).The structures of benzimidazole-fused 12 and tetrahydroquinoline-fused pyrrolo [3,4-b]quinolines 13 were designed based on the predicted mechanism of action of camptothecin on topoisomerase I DNA using the docking of decarbonylated luotonin A onto the topo I.The target compounds 12 and 13 were synthesised from readily available 1-propargyl-benzimidazole-2-carbaldehyde 11a [22] and 1-propargyl-tetrahydroquinoline 11b [23].Thus, boron trifluoride diethyl etherate catalysed intramolecular Povarov reactions using various substituted arylamines provided desired products in good to excellent yields.In 2019, Muthukrishnan and coworkers [24] reported on the novel formal synthes 13-aryl-substituted luotonin A derivatives.The key intermediates of Yadav and Reddy s thesis [25], quinoline fused lactams 15, were obtained from alkyne tethered N-benzyl gly derivatives 14 by oxone-promoted intramolecular dehydrogenative Povarov cyclisation the subsequent deprotection of an amide under acidic conditions (Scheme 4).Prepared de-carbonyl analogues of luotonin A 12 were evaluated for their topoisomerase I inhibition, as well as their in vitro cytotoxic activity against four highly aggressive human cancer cell lines of different origins (KB, a subline of the HeLa cell line, MDA-MB231, LNCap, and HT1080).The 2-methoxy and 4-methoxy derivatives exhibited a high topo I inhibition activity that is comparable to that of the standard drug camptothecin.In the cytotoxicity assay towards several highly aggressive tumours, it was observed that some of the newly prepared luotonin A analogues showed promising activity.Specifically, the 4-Cl-analogue showed improved cytotoxicity towards the KB cell line compared to the parent alkaloid luotonin A.
In 2019, Muthukrishnan and coworkers [24] reported on the novel formal synthesis of 13-aryl-substituted luotonin A derivatives.The key intermediates of Yadav and Reddy synthesis [25], quinoline fused lactams 15, were obtained from alkyne tethered N-benzyl glycine derivatives 14 by oxone-promoted intramolecular dehydrogenative Povarov cyclisation and the subsequent deprotection of an amide under acidic conditions (Scheme 4).In 2019, Muthukrishnan and coworkers [24] reported on the novel formal synthesis o 13-aryl-substituted luotonin A derivatives.The key intermediates of Yadav and Reddy syn thesis [25], quinoline fused lactams 15, were obtained from alkyne tethered N-benzyl glycine derivatives 14 by oxone-promoted intramolecular dehydrogenative Povarov cyclisation and the subsequent deprotection of an amide under acidic conditions (Scheme 4).Recently, a rapid synthesis of luotonin A derivatives using eosin Y photocatalyst and TsOH as a co-catalyst via the Povarov cycloaddition reaction was described [26].The au thors proposed a tentative mechanism for this visible-light-induced transformation (Scheme 5).The intermediate 18 is formed via an intramolecular [4+2] cyclisation of the in situ-generated imine 17 prepared from various anilines and carbaldehydes 16.The result ing cycloadduct 18 is then oxidised using a system consisting of oxygen and an organi dye eosin Y via SET mechanism, forming the quinoline skeleton of the desired luotonin A derivatives.Recently, a rapid synthesis of luotonin A derivatives using eosin Y photocatalyst and TsOH as a co-catalyst via the Povarov cycloaddition reaction was described [26].The authors proposed a tentative mechanism for this visible-light-induced transformation (Scheme 5).The intermediate 18 is formed via an intramolecular [4+2] cyclisation of the in situ-generated imine 17 prepared from various anilines and carbaldehydes 16.The resulting cycloadduct 18 is then oxidised using a system consisting of oxygen and an organic dye eosin Y via SET mechanism, forming the quinoline skeleton of the desired luotonin A derivatives.In 2016, Cheon and coworkers [27] developed a new synthesis of luotonin A starting from ethyl-2-aminocinnamate 19 and quinazolinone-2-carbaldehyde 20 in a 33% total yield over six consecutive steps (Scheme 6).The synthetic strategy is based on two key reactions for the successive production of the B and C rings of target luotonin A. The quinoline scaffold containing the B ring of 1 was formed by 6π electrocyclisation of aldimine 21 in 1,2,4-trichlorobenzene under microwave irradiation.Another key intermediate-alcohol 24-was obtained in three steps involving the basic hydrolysis of ester 22 to carboxylic acid 23, followed by chlorination with oxalyl chloride and reduction using NaBH 4 .Finally, the total synthesis of luotonin A was completed by the formation of a pyrrole C ring using an intramolecular Mitsunobu reaction of alcohol 24.
Scheme 5. Synthesis of luotonin A derivatives via synergistic visible-light photoredox and acid catalysis.
In 2016, Cheon and coworkers [27] developed a new synthesis of luotonin A starting from ethyl-2-aminocinnamate 19 and quinazolinone-2-carbaldehyde 20 in a 33% total yield over six consecutive steps (Scheme 6).The synthetic strategy is based on two key reactions for the successive production of the B and C rings of target luotonin A. The quinoline scaffold containing the B ring of 1 was formed by 6π electrocyclisation of aldimine 21 in 1,2,4-trichlorobenzene under microwave irradiation.Another key intermediate-alcohol 24-was obtained in three steps involving the basic hydrolysis of ester 22 to carboxylic acid 23, followed by chlorination with oxalyl chloride and reduction using NaBH4.Finally, the total synthesis of luotonin A was completed by the formation of a pyrrole C ring using an intramolecular Mitsunobu reaction of alcohol 24.

Synthesis of Luotonins A via Construction of the Pyridine Core (B Ring)
Friedländerʾs synthesis of quinolines has been applied in the synthetic strategies by Menéndez [31] and Rassapalli [32].The condensation between o-amino-benzaldehydes/ketones and corresponding cyclic ketone 31 under acidic conditions (cerium(IV) ammonium nitrate [31] or p-toulenesulfonic acid [32]) provided luotonin A derivatives (Scheme 9).The key lactone 31 was obtained from deoxyvasicinone [31] and isatoic anhydride [32], respectively.The A ring-modified analogues, 2-chloro-14-phenyl and 14-(3,5dimethylphenyl)-substituted luotonin A, showed higher topo I inhibition activity compared to the reference compound 1.Additionally, the 3-methyl-14-(3,5-dimethylphenyl) derivative of 1 exhibited activity comparable to camptothecin.Furthermore, almost all compounds showed better activity than luotonin A in cell cytotoxicity assays [31].Docking studies of luotonin A-topoisomerase I DNA ternary complexes revealed that most compounds bound in a similar manner to standard topoisomerase poisons such as topotecan and luotonin A. In most cases, the presence of the substituent on the B ring at the C-14 position induced a better fit to the binding site.

Synthesis of Luotonins A via Construction of the Pyridine Core (B Ring)
Friedländer's synthesis of quinolines has been applied in the synthetic strategies by Menéndez [31] and Rassapalli [32].The condensation between o-amino-benzaldehydes/ ketones and corresponding cyclic ketone 31 under acidic conditions (cerium(IV) ammonium nitrate [31] or p-toulenesulfonic acid [32]) provided luotonin A derivatives (Scheme 9).The key lactone 31 was obtained from deoxyvasicinone [31] and isatoic anhydride [32], respectively.The A ring-modified analogues, 2-chloro-14-phenyl and 14-(3,5-dimethylphenyl)substituted luotonin A, showed higher topo I inhibition activity compared to the reference compound 1.Additionally, the 3-methyl-14-(3,5-dimethylphenyl) derivative of 1 exhibited activity comparable to camptothecin.Furthermore, almost all compounds showed better activity than luotonin A in cell cytotoxicity assays [31].Docking studies of luotonin A-topoisomerase I DNA ternary complexes revealed that most compounds bound in a similar manner to standard topoisomerase poisons such as topotecan and luotonin A. In most cases, the presence of the substituent on the B ring at the C-14 position induced a better fit to the binding site.In 2020, Chusov et al. [33] described the reductive condensation of o-nitrobenzaldehydes with amines utilising iron pentacarbonyl as a reducing agent.Subsequent oxidation of the intermediate led to a great variety of polycyclic nitrogen-containing heterocycles under mild conditions (Scheme 10).This transformation sequence was also employed in a concise synthesis of luotonin A starting from readily available materials such as 3-pyrrolidinole and 2-nitrobenzaldehyde in three operational steps without chromatography.However, the natural alkaloid was isolated in a low overall yield (2% with a 15% yield for the final step).Scheme 10.A short synthesis of luotonin A.

Synthesis of Luotonins A, B, and E via Construction of the Pyrrole Core (C Ring)
During the reviewed period, several synthetic strategies of luotonins A, B, and E were presented, involving the formation of the pyrrole ring by cyclisation reactions.
Nagarapu and coworkers [34] demonstrated a novel approach for the synthesis of 2dihydroquinazolin-2-ylquinoline 32 using tetrabutylammonium hydrogen sulphate (TBAHS) as an acidic phase-transfer catalyst.Following oxidation of prepared intermediate 32 using KMnO4 provided 2-(4-oxo-3,4-dihydro-quinazolin-2-yl)-quinoline-3-carboxylic acid ethyl ester 22, which was reduced with NaBH4, yielding alcohol 24 (Scheme 11).The pentacyclic skeleton of 1 was accomplished via Mitsunobu cyclisation of alcohol 24.Alternatively, quinoline derivative 22 was also prepared by iodine-mediated electrochemical C(sp 3 )-H cyclisation [35].Luotonin B 2 was prepared by the PCC oxidation of intermediate 24 in CH2Cl2 at a 65% yield.The subsequent treatment of compound 2 with PTSA in methanol provided luotonin E 3 at an 82% yield.Overall, the syntheses of luotonin A, B, and E were accomplished in a direct fashion with high overall yields (57%, 45%, and 37%, respectively).In 2020, Chusov et al. [33] described the reductive condensation of o-nitrobenzaldehydes with amines utilising iron pentacarbonyl as a reducing agent.Subsequent oxidation of the intermediate led to a great variety of polycyclic nitrogen-containing heterocycles under mild conditions (Scheme 10).This transformation sequence was also employed in a concise synthesis of luotonin A starting from readily available materials such as 3-pyrrolidinole and 2-nitrobenzaldehyde in three operational steps without chromatography.However, the natural alkaloid was isolated in a low overall yield (2% with a 15% yield for the final step).In 2020, Chusov et al. [33] described the reductive condensation of o-nitrobenzaldehydes with amines utilising iron pentacarbonyl as a reducing agent.Subsequent oxidation of the intermediate led to a great variety of polycyclic nitrogen-containing heterocycles under mild conditions (Scheme 10).This transformation sequence was also employed in a concise synthesis of luotonin A starting from readily available materials such as 3-pyrrolidinole and 2-nitrobenzaldehyde in three operational steps without chromatography However, the natural alkaloid was isolated in a low overall yield (2% with a 15% yield for the final step).Scheme 10.A short synthesis of luotonin A.

Synthesis of Luotonins A, B, and E via Construction of the Pyrrole Core (C Ring)
During the reviewed period, several synthetic strategies of luotonins A, B, and E were presented, involving the formation of the pyrrole ring by cyclisation reactions.

Synthesis of Luotonins A, B, and E via Construction of the Pyrrole Core (C Ring)
During the reviewed period, several synthetic strategies of luotonins A, B, and E were presented, involving the formation of the pyrrole ring by cyclisation reactions.
The SAR studies revealed that the in vitro anticancer activity of luotonin A was significantly improved by the introduction of 8-piperazine group In 2021, Luo et al. [36] published the structure-activity relationship study of 8,9disubstituted luotonin A analogues and their antiproliferative activity against four cancer cell lines.A series of 8-dialkylamino-9-fluoro luotonin A analogues were prepared in six steps, as outlined in Scheme 12.The quinazolinone intermediate was prepared via an I 2 -catalysed oxidative reaction from 2-amino-4,5-difluorobenzamide and ethyl 2methylquinoline-3-carboxylate in the presence of TsOH in DMSO.Following the reaction sequence, the basic hydrolysis of the ester group, chlorination using oxalyl chloride, reduction with sodium borohydride in THF, and cyclisation under Mitsunobu reaction conditions yielded 8,9-difluoro-luotonin A in 32% yield over five steps [27].Subsequent regioselective nucleophilic substitution with various amines provided the targeted 8-dialkylamino-9fluoro luotonin A analogues in 40-78% yields.5-Deaza luotonin A analogues 34 and 35 were prepared using the same synthetic route from 2-aminobenzamides 33 and 2,3naphthalenedicarboxylic anhydride.
The SAR studies revealed that the in vitro anticancer activity of luotonin A was significantly improved by the introduction of 8-piperazine group Pal and coworkers [37] developed a general synthesis of the quinazolin-4(3H)-onebased alkaloids, including luotonins B and E. The key intermediate, 2-quinolin-2-yl-3Hquinazolin-4-one 36, containing the A, B, D, and E rings of the target compounds, was obtained via a catalyst-free three-component reaction using isatoic anhydride, quinoline-2-carbaldehyde, and formamide (Scheme 13).Following the ortho-lithiation of 36 with mesityllithium, the treatment of in situ-formed lithiated species with DMF afforded luotonin B 2. The described reaction sequence proceeded via intermediary formed aldehyde 38, which directly cyclised, forming a pyrrole ring of luotonin B. Luotonin E was prepared by the methylation of 2 in the presence of PTSA.
Later, Ramamohan et al. [38] described a similar synthetic procedure to luotonins 2 and 3 by applying an iron(III) chloride-catalysed reaction in the synthesis of quinazolin-4(3H)-ones.Thus, the key intermediate 36 was prepared from quinoline-2-carbonitrile in two steps (Scheme 13).In the first step, the transformation of the cyano-group of the starting material was achieved using aqueous hydroxylamine in ethanol, providing amidoxime 37 at a 91% yield.Following the reaction of a mixture of carboxamidine 37 and isatoic anhydride, using 10 mol % FeCl3 in 1,4-dioxane furnished the desired product 36 at a 93% yield.Later, Ramamohan et al. [38] described a similar synthetic procedure to luotonins 2 and 3 by applying an iron(III) chloride-catalysed reaction in the synthesis of quinazolin-4(3H)ones. Thus, the key intermediate 36 was prepared from quinoline-2-carbonitrile in two steps (Scheme 13).In the first step, the transformation of the cyano-group of the starting material was achieved using aqueous hydroxylamine in ethanol, providing amidoxime 37 at a 91% yield.Following the reaction of a mixture of carboxamidine 37 and isatoic anhydride, using 10 mol % FeCl 3 in 1,4-dioxane furnished the desired product 36 at a 93% yield.
fungal activities against five plant fungi species.Most of these luotonin A analogues exhibited significant fungicidal in vitro activity against Botrytis cinerea with EC50 values less than 1 µg/mL.Among them, fluoro-substituted derivatives 44 showed superior antifungal activity against B. cinerea (EC50 for 4-F-44: 0.036 µg/mL, 8-F-44: 0.050 µg/mL, 2,8-di-F-44: 0.042 µg/mL, 10-F-39: 0.048 µg/mL), which was more potent than boscalid (EC50 = 1.790 µg/mL).Scheme 15.Synthesis of luotonin A analogues.Mondal et al. [41] developed a one-pot protocol to synthesise quinazolinone-fused N-heterocyclic compounds 48, structurally similar to the natural luotonins.The pyrrole ring (C-ring) is formed in a key Pd(OAc)2/AgOAc-promoted intramolecular dehydrogenative cross-coupling reaction of 3-aryl quinazolin-4(3H)-ones 47.These starting quinazolines 47 are readily available in good yields via the benzylation of the N-3 centre of quinazolinone 46 using aryl halides 45 in the presence of K2CO3 in acetone (Scheme 16).In the case of substrate 47, which bears R 2 = NO2 and Br substituents, the cyclisation product 48 was isolated along with the O-Ac product 49 formed by subsequent CH2-N-oxidation.Compound 49 was shown to exhibit the best results in the study of a concentrationdependent cell viability assay on SiHa cells, a human cervical cancer cell line, with an observed IC50 value of 23 µM.
Scheme 15.Synthesis of luotonin A analogues.Mondal et al. [41] developed a one-pot protocol to synthesise quinazolinone-fused N-heterocyclic compounds 48, structurally similar to the natural luotonins.The pyrrole ring (C-ring) is formed in a key Pd(OAc) 2 /AgOAc-promoted intramolecular dehydrogenative cross-coupling reaction of 3-aryl quinazolin-4(3H)-ones 47.These starting quinazolines 47 are readily available in good yields via the benzylation of the N-3 centre of quinazolinone 46 using aryl halides 45 in the presence of K 2 CO 3 in acetone (Scheme 16).In the case of substrate 47, which bears R 2 = NO 2 and Br substituents, the cyclisation product 48 was isolated along with the O-Ac product 49 formed by subsequent CH 2 -N-oxidation.Compound 49 was shown to exhibit the best results in the study of a concentrationdependent cell viability assay on SiHa cells, a human cervical cancer cell line, with an observed IC 50 value of 23 µM.
fungal activities against five plant fungi species.Most of these luotonin A analogues exhibited significant fungicidal in vitro activity against Botrytis cinerea with EC50 values less than 1 µg/mL.Among them, fluoro-substituted derivatives 44 showed superior antifungal activity against B. cinerea (EC50 for 4-F-44: 0.036 µg/mL, 8-F-44: 0.050 µg/mL, 2,8-di-F-44: 0.042 µg/mL, 10-F-39: 0.048 µg/mL), which was more potent than boscalid (EC50 = 1.790 µg/mL).Scheme 15.Synthesis of luotonin A analogues.Mondal et al. [41] developed a one-pot protocol to synthesise quinazolinone-fused N-heterocyclic compounds 48, structurally similar to the natural luotonins.The pyrrole ring (C-ring) is formed in a key Pd(OAc)2/AgOAc-promoted intramolecular dehydrogenative cross-coupling reaction of 3-aryl quinazolin-4(3H)-ones 47.These starting quinazolines 47 are readily available in good yields via the benzylation of the N-3 centre of quinazolinone 46 using aryl halides 45 in the presence of K2CO3 in acetone (Scheme 16).In the case of substrate 47, which bears R 2 = NO2 and Br substituents, the cyclisation product 48 was isolated along with the O-Ac product 49 formed by subsequent CH2-N-oxidation.Compound 49 was shown to exhibit the best results in the study of a concentrationdependent cell viability assay on SiHa cells, a human cervical cancer cell line, with an observed IC50 value of 23 µM.Scheme 16.Synthesis of luotonin A analogues [33].Scheme 16.Synthesis of luotonin A analogues [33].In 2018, Toyota and coworkers [42] presented an improved synthesis of luotonin A and its analogues based on previous results [43,44].Similarly, the construction of the pyrrolo[1,2-a]pyrimidinone skeleton (C and D rings) was achieved by anion-assisted double intramolecular hetero Diels-Alder transformation.The reaction of amides 50 using Pd 2 (dba) 3 as a catalyst and DPPF as a ligand together with a mixture of CuCN and Et 4 NCN as a cyanide donor at 130 • C in dioxane provided the desired pentacyclic compounds in good yields (Scheme 17).

Synthesis of Luotonin
and its analogues based on previous results [43,44].Similarly, the construction of the pyrrolo[1,2-a]pyrimidinone skeleton (C and D rings) was achieved by anion-assisted double intramolecular hetero Diels-Alder transformation.The reaction of amides 50 using Pd2(dba)3 as a catalyst and DPPF as a ligand together with a mixture of CuCN and Et4NCN as a cyanide donor at 130 °C in dioxane provided the desired pentacyclic compounds in good yields (Scheme 17).Scheme 17. Synthesis of luotonin A and its analogues via intramolecular Diels-Alder reaction.
Evano and coworkers [45] described a concise synthesis of luotonin A, in which the pyrrolopyrimidinone fragment (C and D rings) of the pentacyclic skeleton of 1 was constructed in a single step using cooper-catalysed radical domino reaction of cyanamide 53.The corresponding N-benzoylcyanamide 53 was prepared using a two-step sequence from 2-iodo-3-aminomethylquinoline 52 [46] involving the reaction using cyanogen bromide followed by a benzoylation step (Scheme 18).Thus, the treatment of 53 with 10 mol% of heteroleptic copper(I) complex [(DPEphos)(bcp)Cu]PF6 in the presence of dicyclohexylisobutylamine as the reductant and K2CO3 as a base under light irradiation (420 nm) in acetonitrile at room temperature afforded luotonin A (1) at a 79% The mechanism of this photoinduced domino transformation involves the generation of radical intermediate 54, which undergoes 5-exo-dig cyclisation to form the intermediate iminyl radical 55.This radical 55 then cyclises through a 6-endo-trig process and subsequent aromatisation of in situ-formed 56 provides luotonin A (1). Evano and coworkers [45] described a concise synthesis of luotonin A, in which the pyrrolopyrimidinone fragment (C and D rings) of the pentacyclic skeleton of 1 was constructed in a single step using cooper-catalysed radical domino reaction of cyanamide 53.The corresponding N-benzoylcyanamide 53 was prepared using a two-step sequence from 2-iodo-3-aminomethylquinoline 52 [46] involving the reaction using cyanogen bromide followed by a benzoylation step (Scheme 18).Thus, the treatment of 53 with 10 mol% of heteroleptic copper(I) complex [(DPEphos)(bcp)Cu]PF 6 in the presence of dicyclohexylisobutylamine as the reductant and K 2 CO 3 as a base under light irradiation (420 nm) in acetonitrile at room temperature afforded luotonin A (1) at a 79% yield.The mechanism of this photoinduced domino transformation involves the generation of radical intermediate 54, which undergoes 5-exo-dig cyclisation to form the intermediate iminyl radical 55.This radical 55 then cyclises through a 6-endo-trig process and subsequent aromatisation of in situ-formed 56 provides luotonin A (1).
and its analogues based on previous results [43,44].Similarly, the construction of the pyrrolo[1,2-a]pyrimidinone skeleton (C and D rings) was achieved by anion-assisted double intramolecular hetero Diels-Alder transformation.The reaction of amides 50 using Pd2(dba)3 as a catalyst and DPPF as a ligand together with a mixture of CuCN and Et4NCN as a cyanide donor at 130 °C in dioxane provided the desired pentacyclic compounds in good yields (Scheme 17).Scheme 17. Synthesis of luotonin A and its analogues via intramolecular Diels-Alder reaction.
Evano and coworkers [45] described a concise synthesis of luotonin A, in which the pyrrolopyrimidinone fragment (C and D rings) of the pentacyclic skeleton of 1 was constructed in a single step using cooper-catalysed radical domino reaction of cyanamide 53.The corresponding N-benzoylcyanamide 53 was prepared using a two-step sequence from 2-iodo-3-aminomethylquinoline 52 [46] involving the reaction using cyanogen bromide followed by a benzoylation step (Scheme 18).Thus, the treatment of 53 with 10 mol% of heteroleptic copper(I) complex [(DPEphos)(bcp)Cu]PF6 in the presence of dicyclohexylisobutylamine as the reductant and K2CO3 as a base under light irradiation (420 nm) in acetonitrile at room temperature afforded luotonin A (1) at a 79% yield.The mechanism of this photoinduced domino transformation involves the generation of radical intermediate 54, which undergoes 5-exo-dig cyclisation to form the intermediate iminyl radical 55.This radical 55 then cyclises through a 6-endo-trig process and subsequent aromatisation of in situ-formed 56 provides luotonin A (1). Scheme 18. Evanoʾs synthesis of luotonin A via photoinduced Cu-catalysed cyclisation.Scheme 18. Evano's synthesis of luotonin A via photoinduced Cu-catalysed cyclisation.

Summary and Perspective
This review presents the syntheses and biological properties of new derivatives of luotonins A, B, and E. Many new analogues of these natural products, bearing various substituents at different positions on the A, B, C and E rings, have been reported.Some of these derivatives were found to exhibit enhanced topoisomerase I inhibition and anticancer, antifungal, and antiviral activities compared to naturally occurring luotonin A. The broad spectrum of biological properties suggests the potential of these analogues, prompting further systematic study of such molecules and their structural analogues.During the period covered in this review, synthetic strategies based mostly on the Povarov [4+2] cycloaddition and aza-Nazarov-Frieländer cyclisation reaction were used for the construction of the 5H-pyrrolo [4,3-b]pyridine core (B and C rings).The next part of this review discusses advancements in the synthesis of the pentacyclic skeleton of 1 involving the pyrrole ring formation (C ring).Additionally, synthetic methods forming the pyrrolo[1,2-a]pyrimidin-4(6H)-one fragment (C and D rings) provide access to the desired quinolino[2 ′ ,3 ′ :3,4]pyrrolo[2,1-b]quinazolin-11(13H)-one scaffold.These approaches typically involve multistep procedures or complex substrates.The main challenge remains to develop an effective strategy for the construction of a pentacyclic backbone by the simultaneous formation of more bonds in a single step.It is anticipated that this could be achieved by exploiting the potential of a Heck-type reaction in new domino sequences, starting from readily/commercially available substrates.Another significant challenge lies in developing suitable methods for industrial applications, e.g., scalable or continuous flow synthesis.

Figure 1 .
Figure 1.Structures of luotonins A, B, and E and camptothecin.

Figure 1 .
Figure 1.Structures of luotonins A, B, and E and camptothecin.

Figure 2 .
Figure 2. Structures of A and E substituted analogues of luotonin A.

Figure 2 .
Figure 2. Structures of A and E substituted analogues of luotonin A.

Figure 2 .
Figure 2. Structures of A and E substituted analogues of luotonin A.

Scheme 6 . 17 Scheme 8 .
Scheme 6. Cheon's synthesis of luotonin A.Another approach to 12-aryl-substituted luotonins A and their regioisomeric analogues 26 via an acid-mediated aza-Nazarov-Friedländer condensation sequence using quinazolinonyl enones 25 was developed by Rassapali and coworkers[28,29]  (Scheme 7).The regioselectivity of the Nazarov cyclisation was successfully controlled by the acidic Scheme 6. Cheon's synthesis of luotonin A.Another approach to 12-aryl-substituted luotonins A and their regioisomeric analogues 26 via an acid-mediated aza-Nazarov-Friedländer condensation sequence using quinazolinonyl enones 25 was developed by Rassapali and coworkers[28,29]  (Scheme 7).The regioselectivity of the Nazarov cyclisation was successfully controlled by the acidic reaction conditions.The test for cytotoxicity and topo I inhibition showed only weak activities compared to adriamycin and camptothecin, respectively.Molecules 2024, 29, x FOR PEER REVIEW 7 of 17

Scheme 8 .
Scheme 8. Synthesis of luotonin A, B, and E derivatives.